Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples

[EN] Currently, the techniques used to analyse fish spermatozoa head morphology are not only subjective and timeconsuming, but also, variable due to alteration of real spermatozoa size during sample manipulation. Thereby, it is important to develop a new method to obtain accurate and objective resul...

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Bibliographic Details
Published in:Aquaculture
Main Authors: Caldeira, Carina, Hernández-Ibánez, Sandra, Vendrell, Alberto, Valverde, Anthony, Garcia-Molina, Almudena, Gallego Albiach, Victor, Asturiano, Juan F., Soler, Carles
Other Authors: Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, COMISION DE LAS COMUNIDADES EUROPEA, Ministerio de Ciencia, Innovación y Universidades, Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica, Consejo Nacional para Investigaciones Científicas y Tecnológicas, Costa Rica
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2022
Subjects:
CASA-Morph system
Sperm morphometry characterisation
Fixation solution
TruMorph® tool
Pressure
Realistic measurements
PRODUCCION ANIMAL
14.- Conservar y utilizar de forma sostenible los océanos
mares y recursos marinos para lograr el desarrollo sostenible
Cierva
Molina
Anguilla anguilla
Online Access:http://hdl.handle.net/10251/193396
https://doi.org/10.1016/j.aquaculture.2022.738047
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Summary:[EN] Currently, the techniques used to analyse fish spermatozoa head morphology are not only subjective and timeconsuming, but also, variable due to alteration of real spermatozoa size during sample manipulation. Thereby, it is important to develop a new method to obtain accurate and objective results. So far, computer-assisted systems for morphometry analysis were developed and validated for mammals, although they could also be adapted to fish spermatozoa. The present study aimed to characterise the European eel spermatozoa morphometry, comparing the measurements (area and perimeter) obtained by computer-assisted spermatozoa between pretreated or non-treated eel sperm samples (n = 5) with fixation solution. In both protocols, the TruMorph (R) tool was used to apply a constant force to extend the cells in a thin layer. Images of spermatozoa were captured using a 40x negative phase contrast objective and analysed with the ISASv1 CASA-Morph system. Sperm head morphometry showed significant differences in area and perimeter comparing both protocols. Besides, the head size analysis using TruMorph (R) without fixation along time showed that the sperm membrane remains intact with the use of this technique, preserving the semipermeable condition. Considering the fact that fixation solution produces dehydration/hydration that could affect the real spermatozoa size, the simple use of TruMorph (R) without fixation combined with CASA-Morph analysis could allow more realistic measurements of eel spermatozoa head. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N. 642893 (IMPRESS). AV is granted by the CONICIT and MICITT, Costa Rica. VG has a post-doc contract from the Spanish Ministry of Innovation, Science and Universities (Juan de la Cierva-Incorporaci ' on; IJCI-2017-34200). Caldeira, C.; Hernández-Ibánez, S.; Vendrell, A.; Valverde, A.; Garcia-Molina, A.; Gallego Albiach, V.; Asturiano, JF. (2022). ...

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