Dietary amino acids impact sperm performance traits for a catadromous fish, Anguilla anguilla reared in captivity

• Published in: Aquaculture
• Main Authors: Butts, Ian Anthony Ernest, Hilmarsdóttir, Gudrun Svana, Zadmajid, Vahid, Gallego Albiach, Victor, Stottrup, Josianne G., Jacobsen, Charlotte, Krüger-Johnsen, Maria, Politis, Sebastian N., Asturiano, Juan F., Holst, Lars K., Tomkiewicz, Jonna
• Other Authors: Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal, Innovation Fund Denmark, Agencia Estatal de Investigación, National Institute of Food and Agriculture, EEUU
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2020
• Subjects: Aquaculture; Broodstock diet; Assisted reproduction; Gamete quality; European eel; PRODUCCION ANIMAL; Anguilla anguilla
• Online Access: http://hdl.handle.net/10251/165301; https://doi.org/10.1016/j.aquaculture.2019.734602

Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. [EN] Little is known about the role of dietary amino acids on male reproductive performance and gamete quality in fishes. Thus, the objective of this study was to investigate how "enhanced" feeds (EH-4, EH-5, EH-6), with modified amino acid composition, and the standard on-growing diet (DAN-EX) impact body composition, milt biochemistry, and sperm performance in male European eel, Anguilla anguilla. The fatty acid composition of EH4, EH-5, and EH-6 was similar but differed to that in DAN-EX, while amino acid composition varied between all four diets. Diet did not influence organ-somatic indices (e.g. HSI, GSI), while males fed EH-4 were heavier than other groups. Arginine, alanine, and lysine were the most abundant amino acids in milt (>11%), followed by glycine, aspartic acid, valine, glutamic acid, and leucine ( >5.66%). Diet impacted milt arginine, serine, proline, methionine, and histidine levels. Specifically, males fed DAN-EX, EH-4, and EH-5 had the highest percentages of arginine, while males fed EH-4 to EH-6 had higher percentages of serine. Proline was most abundant in males fed DAN-EX, EH-5, and EH-6. Both methionine and histidine were detected at low percentages ( 2%), and were impacted by diet, where males fed EH-4 and EH-5 had higher percentages of methionine, and males fed DAN-EX, EH-4, and EH-6 had the highest percentage of histidine. Milt production increased over time, where eels fed EH4 and EH-6 showed the highest probability of producing suited milt volumes ( > 0.5 mL) for fertilization procedures. Spermatocrit (43.1 +/- 1.80%) did not differ between the diets (ranged from 37.57 to 47.21%). Dietary regime had an impact on sperm motility, such that eels fed EH-5 and EH-6 had the greatest percentage of motile cells. In addition, fish fed EH-5 and EH-6 (or DAN-EX) had the fastest swimming sperm. ...