First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system

The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (V...

Full description

Bibliographic Details
Published in:Aquaculture
Main Authors: de Decker, Sophie, Reynaud, Yann, Saulnier, Denis
Other Authors: Unité Santé, Génétique et Microbiologie des Mollusques (SGMM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM), Institut de Recherche pour le Développement (IRD), Unité Microbiologie Aliment Santé Environnement (MASAE)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2013
Subjects:
[SDV]Life Sciences [q-bio]
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://hal.science/hal-04499316
https://doi.org/10.1016/j.aquaculture.2013.01.033
Description
Summary:The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependentmetalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam were performed. A relative increase in expressionwas observed for vsm and, particularly, vam in the presence of the conditioned medium, probably controlled by a quorumsensing system. The study revealed intraspecific and interspecific modulation of expression in metalloprotease genes vsm and vam in V. splendidus and V. aestuarianus. Our experiments showed for the first time that V. splendidus is able to produce an autoinducer-like substance that displays intra- and interspecific effects on the expression of two different metalloprotease genes: vsm and vam

Similar Items