High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

• Main Authors: Mat Radzi, Salina, Basri, Mahiran, Salleh, Abu Bakar, Ariff, Arbakariya, Mohammad, Rosfarizan, Abdul Rahman, Mohd Basyaruddin, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Pontificia Universidad Católica de Valparaíso 2005
• Subjects: antartic*
• Online Access: http://psasir.upm.edu.my/7056/; http://psasir.upm.edu.my/7056/1/High%20performance%20enzymatic%20synthesis%20of%20oleyl%20oleate%20using%20immobilised%20lipase%20from%20Candida%20antartica.pdf; http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n3-4

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.