Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system
Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to...
| Main Authors: | , , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Springer
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/15995/ http://psasir.upm.edu.my/15995/1/Optimized%20lipase%20catalyzed%20synthesis%20of%20adipate%20ester%20in%20a%20solvent.pdf |
| Summary: | Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester. |
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