Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate,...

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Bibliographic Details
Main Authors: Abd. Shukor, Mohd. Yunus, Ali Hassan, Nor Ayshah Alia, Jusoh, Arif Zaidi, Nagar, Perumal, Shamaan, Nor Aripin, Mac Cormack, Walter Patricio, Syed, Mohd Arif
Format: Article in Journal/Newspaper
Language:English
Published: Triveni Enterprises 2009
Subjects:
Antarc*
Antarctica
Antarctica Journal
Online Access:http://psasir.upm.edu.my/15320/
http://psasir.upm.edu.my/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf
http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl
Description
Summary:A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.

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