INVESTIGATION OF THE INTRACELLULAR BINDING SITE OF CAROTENOIDS IN THE SKELETAL MUSCLE OF SALMONIDS
The intracellular binding site of carotenoids in the skeletal muscle of Atlantic salmon (Salmo salar) and Rainbow trout (Onchorhynchus mykiss) was investigated. The hypothesis tested was carotenoids are bound to the mitochondrial fraction of the muscle. Muscle samples were homogenized, the organelle...
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| Format: | Thesis |
| Language: | English |
| Published: |
2002
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| Online Access: | http://hdl.handle.net/10026.2/2119 |
| Summary: | The intracellular binding site of carotenoids in the skeletal muscle of Atlantic salmon (Salmo salar) and Rainbow trout (Onchorhynchus mykiss) was investigated. The hypothesis tested was carotenoids are bound to the mitochondrial fraction of the muscle. Muscle samples were homogenized, the organelles were separated by differential centrifugation into different fractions and protein and carotenoid content measurements were performed. The presence of mitochondria was assayed by the activity of Succinate Dehydrogenase (SDH). Identification of carotenoids was carried out by HPLC and were found to be astaxanthin for salmon muscle and canthaxanthin for trout muscle. For every organelle fraction the SDH specific activity (s.a.) and Carotenoids (Car.) specific content (s.c.) were calculated along with their purification folds. The analysis of variance showed that most of the mitochondria were isolated in a single fraction, P2, that the highest Car. s.c. was present in the sarcoplasmic reticulum (SR) fraction, while the regression analysis showed that there was no correlation between SDH s.a. and Car. s.c. Density gradient centrifugations that were carried out on P2 resulted in separation of carotenoids and mitochondria. The results indicate that the carotenoids in the muscle of salmon and trout are not associated with the mitochondria but with the SR. |
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