F_type lectin from the sea bass (Dicentrarchus Labrax): Purification, cDNA clonig, tissue expression and localization, and opsonic activity

Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: SALERNO, Giuseppina, PARISI, Maria Giovanna, PARRINELLO, Daniela, BENENATI, Gigliola, VIZZINI, Aiti, VAZZANA, Mirella, CAMMARATA, Matteo, Vasta, GR
Other Authors: Salerno, G, Parisi, MG, Parrinello, D, Benenati, G, Vizzini, A, Vazzana, M, Cammarata, M
Format: Article in Journal/Newspaper
Language:English
Published: Academic Press. 2009
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Online Access:http://hdl.handle.net/10447/35089
https://doi.org/10.1016/j.fsi.2009.01.004
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Summary:Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. The F-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. Here we describe the purification, biochemical and molecular properties, and the opsonic activity of an F-type lectin (DlFBL) isolated from sea bass (Dicentrarchus labrax) serum. DlFBL exhibits two tandemly arranged carbohydrate-recognition domains that display the F-type sequence motif. In situ hybridization and immunohistochemical analysis revealed that DlFBL is specifically expressed and localized in hepatocytes and intestinal cells. Exposure of formalin-killed Escherichia coli to DlFBL enhanced their phagocytosis by D. labrax peritoneal macrophages relative to the unexposed controls, suggesting that DlFBL may function as an opsonin in plasma and intestinal mucus.