Population genetic variation of the Southern Ocean krill, Euphausia superba, in the Western Antarctic Peninsula region based on mitochondrial single nucleotide polymorphisms (SNPs)

• Published in: Deep Sea Research Part II: Topical Studies in Oceanography
• Main Authors: P. G. Batta Lona, A. Bucklin, P. H. Wiebe, N. J. Copley, PATARNELLO, TOMASO
• Other Authors: P. G., Batta Lona, A., Bucklin, P. H., Wiebe, Patarnello, Tomaso, N. J., Copley
• Format: Article in Journal/Newspaper
• Language: English
• Published: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND 2011
• Subjects: Euphausia superba; Population genetic; Single nucleotide polymorphism; Southern Ocean krill; Antarctic; Southern Ocean; Antarctic Peninsula; Austral; Marguerite; Marguerite Bay; Crystal Sound; Antarc*
• Online Access: http://hdl.handle.net/11577/2489697; https://doi.org/10.1016/j.dsr2.2010.11.017

The Southern Ocean krill, Euphausia superba, is one of the best-studied marine zooplankton species in terms of population genetic diversity and structure; with few exceptions, previous studies have shown the species to be genetically homogeneous at larger spatial scales. The goals of this study are to examine sub-regional scale population genetic diversity and structure of E. superba using molecular characters selected with this goal in mind, and to thereby examine hypotheses of the source(s) of recruitment for krill populations of the Western Antarctic Peninsula (WAP). Collections were made throughout the WAP region during US GLOBEC cruises in austral fall, 2001 and 2002. A total of 585 E. superba (including all 6 furcilia larval stages, juveniles, and adults) was analyzed after confirmation of species identification using a competitive multiplexed species-specific PCR (SS-PCR) reaction based on mitochondrial cytochrome oxidase I (mtCOI) sequences. The molecular markers used were allele frequencies at single nucleotide polymorphism (SNP) sites in the gene encoding mitochondria! Cytochrome b (cyt 6). Four SNP sites that showed desirable patterns of allelic variation were selected; alleles were detected using a multiplexed single-base extension PCR protocol. A total of 22 SNP haplotypes (i.e., strings of polymorphisms at the four SNP sites) was observed; haplotype diversity (Hd)=0.811 (s.d.=0.008). Analysis of molecular variation within and among samples, areas (i.e., Marguerite Bay, Crystal Sound, shelf, and offshore) and collection years revealed no difference between 2001 and 2002 collections overall, although differences between 2001 and 2002 collections from Marguerite Bay explained 7.4% of the variance (F(ST)=0.072; p=0.002 +/- 0.001). Most of the variation (96.3%) occurred within samples each year, with no significant differentiation among areas. There was small, but significant differentiation among samples within areas in 2001 (4.6%; F(ST)=0.045; p=0.015 +/- 0.003) and 2002 (6.3%; F(ST)=0.062; p=0.000 ...