A rapid and high-throughput real-time PCR assay for species identification: Application to stockfish sold in Italy

In some parts of Italy, there is a tradition of eating special, highly prized species of cod, fished and dried in Norway. In order to safeguard the value of this food product, in 2005, the Italian government legislated that the commercial term "stockfish" can only be attributed to Gadus mo...

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Bibliographic Details
Published in:European Food Research and Technology
Main Authors: BERTOJA G, GIACCONE, VALERIO, CARRARO, LISA, MININNI, ALBA NICOLETTA, CARDAZZO, BARBARA
Other Authors: Bertoja, G, Giaccone, Valerio, Carraro, Lisa, Mininni, ALBA NICOLETTA, Cardazzo, Barbara
Format: Article in Journal/Newspaper
Language:English
Published: 2009
Subjects:
Norway
Gadus morhua
Online Access:http://hdl.handle.net/11577/2376931
https://doi.org/10.1007/s00217-009-1038-8
Description
Summary:In some parts of Italy, there is a tradition of eating special, highly prized species of cod, fished and dried in Norway. In order to safeguard the value of this food product, in 2005, the Italian government legislated that the commercial term "stockfish" can only be attributed to Gadus morhua (Gm). In this study, we present an improved real-time PCR assay for efficient identification of Gm with respect to other gadiforms of commercial interest. The method is applied to 437 stockfish samples, collected in various Italian regions, in order to verify whether labelling regulations had been respected and to report instances of fraud in the Italian stockfish market. The PCR method employed here allowed rapid and economical identification of the species, with a very high percentage of correct identifications.

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