Isolation and characterization of eight microsatellite loci in the icefish Chaenocephalus aceratus (Perciformes, Notothenioidea, Channichthyidae)

We characterized eight polymorphic microsatellites in the Scotia icefish Chaenocephalus aceratus (Perciformes, Notothenioidei, Channichthyidae) that is endemic of Southern Ocean waters surrounding the tip of the Antarctic Peninsula. Microsatellites were isolated from a partial genomic library enrich...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: SUSANA E, BORTOLOTTO E, BUCCOLI S, PAPETTI, CHIARA, BARBISAN, FEDERICA, PATARNELLO, TOMASO, ZANE, LORENZO
Other Authors: Susana, E, Papetti, Chiara, Barbisan, Federica, Bortolotto, E, Buccoli, S, Patarnello, Tomaso, Zane, Lorenzo
Format: Article in Journal/Newspaper
Language:English
Published: BBLACKWELL PUBLISHING, 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND 2007
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Online Access:http://hdl.handle.net/11577/1777336
https://doi.org/10.1111/j.1471-8286.2007.01703.x
Description
Summary:We characterized eight polymorphic microsatellites in the Scotia icefish Chaenocephalus aceratus (Perciformes, Notothenioidei, Channichthyidae) that is endemic of Southern Ocean waters surrounding the tip of the Antarctic Peninsula. Microsatellites were isolated from a partial genomic library enriched for an AC motif. The number of alleles ranged from two to 19 with a mean observed hererozygosity of 0.71. Loci were in Hardy–Weinberg equilibrium and no evidence for linkage disequilibrium was found. These molecular markers will be useful to investigate Scotia icefish genetic structure, possibly providing insights on its effective population size and demographic history.