Efecto antioxidante por la ingesta de la asociación de Spinacia oleracea “espinaca” con lactobacillus plantarum en hígado de Rattus rattus variedad albinus

In the present work the antioxidant effect was determined by the ingestion of the association of Spinacia oleracea "spinach" with Lactobacillus plantarum in Rattus rattus variety albinus. To carry out the process, the spinach plants were collected and their leaves were treated, the surface...

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Bibliographic Details
Main Author: CHAFLOQUE VARGAS, Jose Miguel
Other Authors: PRETEL SEVILLANO, Orlando Enrique
Format: Bachelor Thesis
Language:Spanish
Published: Universidad Nacional de Trujillo 2019
Subjects:
Online Access:http://dspace.unitru.edu.pe/handle/UNITRU/12458
Description
Summary:In the present work the antioxidant effect was determined by the ingestion of the association of Spinacia oleracea "spinach" with Lactobacillus plantarum in Rattus rattus variety albinus. To carry out the process, the spinach plants were collected and their leaves were treated, the surfaces of the leaves were washed with tap water and degreased with alcohol, then brought to the stove until a fine powder was obtained. , preparation of the suspension of Lactobacillus plantarum ATCC 8014 was made at a concentration of, which was seeded in the MRS culture medium (Man Agar, Rogosa and Sharpe), the rats that were randomly grouped into 6 groups, then obtained the hepatic homogenate to then measure the amount of free radicals by the Thiobarbituric Acid Reactive Substances (TBARS) method, 1g of rat liver in 5ml of Krebs solution was used. When performing the statistical analysis, it was concluded that the antioxidant effect of Spinacia oleracea and Lactobacillus plantarum decreases the antioxidant effect of free radicals. En el presente trabajo se determinó el efecto antioxidante por la ingesta de la asociación de la Spinacia oleracea “espinaca” con Lactobacillus plantarum en Rattus rattus variedad albinus. Para llevar a cabo el proceso se recolecto las plantas de espinaca y se le dió un tratamiento de sus hojas, lavando las superficies de las hojas con agua de caño y desengrasando con alcohol, posteriormente se llevó a la estufa hasta la obtención de un polvo fino, se hizo la preparación de la suspensión de Lactobacillus plantarum ATCC 8014 a una concentración de , que fue sembrada en el medio de cultivo MRS(Agar Man, Rogosa y Sharpe), las ratas que fueron agrupadas al azar en 6 grupos, luego se obtuvo el homogenizado hepático para luego medir la cantidad de radicales libres por el método de las Sustancias Reactivas al Acido Tiobarbiturico (TBARS), se usó 1g de hígado de rata en 5ml de solución de Krebs . Al realizar el análisis estadístico se concluyó que el efecto antioxidante de la Spinacia oleracea y Lactobacillus plantarum disminuyen el efecto antioxidante de los radicales libres. Tesis