Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius)
Transmission electron microscopy of otoliths from the inner ear of arctic charr and pike has revealed the presence of fine banding on the scale of several nanometers. The thickness of the bands was observed to vary in different portions of the sample, and some areas were not banded. EDS analysis cou...
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Oak Ridge National Laboratory
1997
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ftunivnotexas:info:ark/67531/metadc690136 2023-05-15T14:30:04+02:00 Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) Meldrum, A. Halden, N. M. United States. Department of Energy. Office of Energy Research. 1997-12-31 6 p. Text https://digital.library.unt.edu/ark:/67531/metadc690136/ English eng Oak Ridge National Laboratory other: DE98001934 rep-no: ORNL/CP--95553 rep-no: CONF-971201-- grantno: AC05-96OR22464 osti: 565283 https://digital.library.unt.edu/ark:/67531/metadc690136/ ark: ark:/67531/metadc690136 1997 fall meeting of the Materials Research Society, Boston, MA (United States), 1-5 Dec 1997 Layers Biological Materials Aragonite Seasonal Variations Chemical Composition Thickness 55 Biology And Medicine Basic Studies Calcium Carbonates Fishes Electron Diffraction Transmission Electron Microscopy Article 1997 ftunivnotexas 2021-05-29T22:08:31Z Transmission electron microscopy of otoliths from the inner ear of arctic charr and pike has revealed the presence of fine banding on the scale of several nanometers. The thickness of the bands was observed to vary in different portions of the sample, and some areas were not banded. EDS analysis could not detect chemical differences within the bands, but electron diffraction showed that the crystallographic orientation of the bands is related by a lattice mismatch. Previously, banding on the scale of 50 to 100 microns was observed by SEM in otoliths from arctic charr and was attributed to seasonal variations in growth. The fine-scale banding observed in this study, however, is unlikely to represent a daily variation. Electron diffraction from the pike samples shows that the material is composed of CaCO{sub 3} having the both the vaterite and aragonite structure, and hydrous CaCO{sub 3} was also observed. The large-scale banding previously identified by SEM was not observed in the TEM despite attempts to intersect the boundaries of the micron-sized layers. The interaction of the electron beam with the sample material was investigated by conducting several electron-irradiation experiments. The electron beam was observed to interact strongly with the sample and caused the precipitation of cubic CaO from the calcium carbonate matrix. Bright-field imaging showed the development of fine grained ({approximately} 5 nm) randomly oriented crystallites which accumulated with increasing electron dose. These initial results suggest that the precipitation of CaO is not driven by electron-beam beating. Previously, a similar phase-change phenomenon has been observed in hydroxyapatite from dental enamel. Other Ca-bearing biominerals may therefore also be expected to be sensitive to electron irradiation. Article in Journal/Newspaper Arctic charr Arctic Esox lucius University of North Texas: UNT Digital Library Arctic |
institution |
Open Polar |
collection |
University of North Texas: UNT Digital Library |
op_collection_id |
ftunivnotexas |
language |
English |
topic |
Layers Biological Materials Aragonite Seasonal Variations Chemical Composition Thickness 55 Biology And Medicine Basic Studies Calcium Carbonates Fishes Electron Diffraction Transmission Electron Microscopy |
spellingShingle |
Layers Biological Materials Aragonite Seasonal Variations Chemical Composition Thickness 55 Biology And Medicine Basic Studies Calcium Carbonates Fishes Electron Diffraction Transmission Electron Microscopy Meldrum, A. Halden, N. M. Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
topic_facet |
Layers Biological Materials Aragonite Seasonal Variations Chemical Composition Thickness 55 Biology And Medicine Basic Studies Calcium Carbonates Fishes Electron Diffraction Transmission Electron Microscopy |
description |
Transmission electron microscopy of otoliths from the inner ear of arctic charr and pike has revealed the presence of fine banding on the scale of several nanometers. The thickness of the bands was observed to vary in different portions of the sample, and some areas were not banded. EDS analysis could not detect chemical differences within the bands, but electron diffraction showed that the crystallographic orientation of the bands is related by a lattice mismatch. Previously, banding on the scale of 50 to 100 microns was observed by SEM in otoliths from arctic charr and was attributed to seasonal variations in growth. The fine-scale banding observed in this study, however, is unlikely to represent a daily variation. Electron diffraction from the pike samples shows that the material is composed of CaCO{sub 3} having the both the vaterite and aragonite structure, and hydrous CaCO{sub 3} was also observed. The large-scale banding previously identified by SEM was not observed in the TEM despite attempts to intersect the boundaries of the micron-sized layers. The interaction of the electron beam with the sample material was investigated by conducting several electron-irradiation experiments. The electron beam was observed to interact strongly with the sample and caused the precipitation of cubic CaO from the calcium carbonate matrix. Bright-field imaging showed the development of fine grained ({approximately} 5 nm) randomly oriented crystallites which accumulated with increasing electron dose. These initial results suggest that the precipitation of CaO is not driven by electron-beam beating. Previously, a similar phase-change phenomenon has been observed in hydroxyapatite from dental enamel. Other Ca-bearing biominerals may therefore also be expected to be sensitive to electron irradiation. |
author2 |
United States. Department of Energy. Office of Energy Research. |
format |
Article in Journal/Newspaper |
author |
Meldrum, A. Halden, N. M. |
author_facet |
Meldrum, A. Halden, N. M. |
author_sort |
Meldrum, A. |
title |
Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
title_short |
Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
title_full |
Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
title_fullStr |
Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
title_full_unstemmed |
Fine-scale oscillatory banding in otoliths from arctic charr (Salveninus alpinus) and pike (Esox lucius) |
title_sort |
fine-scale oscillatory banding in otoliths from arctic charr (salveninus alpinus) and pike (esox lucius) |
publisher |
Oak Ridge National Laboratory |
publishDate |
1997 |
url |
https://digital.library.unt.edu/ark:/67531/metadc690136/ |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Esox lucius |
genre_facet |
Arctic charr Arctic Esox lucius |
op_source |
1997 fall meeting of the Materials Research Society, Boston, MA (United States), 1-5 Dec 1997 |
op_relation |
other: DE98001934 rep-no: ORNL/CP--95553 rep-no: CONF-971201-- grantno: AC05-96OR22464 osti: 565283 https://digital.library.unt.edu/ark:/67531/metadc690136/ ark: ark:/67531/metadc690136 |
_version_ |
1766303989075279872 |