O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B

Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical...

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Bibliographic Details
Published in:Carbohydrate Research
Main Authors: Di Guida R., Casillo A., Corsaro M. M.
Other Authors: Di Guida, R., Casillo, A., Corsaro, M. M.
Format: Article in Journal/Newspaper
Language:English
Published: 2020
Subjects:
Gulopyranuronamide
Lipopolysaccharide
NMR
Pseudomona
Psychrotolerant
Antarctic
The Antarctic
Antarc*
Online Access:http://hdl.handle.net/11588/817216
https://doi.org/10.1016/j.carres.2020.108125
Description
Summary:Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-L-GulpNAc3OAcAN-(1 →3)-β-D-QuipNAc-(1→.

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