Linear aminolipids with moderate antimicrobial activity from the antarctic gram-negative bacterium aequorivita sp

The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of t...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Chianese G., Esposito F. P., Parrot D., Ingham C., de Pascale D., Tasdemir D.
Other Authors: Chianese, G., Esposito, F. P., Parrot, D., Ingham, C., de Pascale, D., Tasdemir, D.
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
Aequorivita
Gram-negative bacterium
LC-MS/MS
Linear aminolipid
Miniaturized culture chip
MRSA
Amino Acid
Antarctic Region
Anti-Bacterial Agent
Chemical Fractionation
Fatty Acid
Flavobacteriaceae
Geologic Sediment
Metabolomic
Methicillin-Resistant Staphylococcus aureu
Microbial Sensitivity Test
Molecular Structure
Seawater
Sequence Analysis
DNA
Spectroscopy
Fourier Transform Infrared
Antarctic
The Antarctic
Antarc*
Online Access:http://hdl.handle.net/11588/775544
https://doi.org/10.3390/md16060187
http://www.mdpi.com/1660-3397/16/6/187/pdf
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Summary:The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1−3) and four known (4−7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [α]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22−145 μg/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita.

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