Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature

Psychrobacter arcticus 273-4 is a Gram-negative bacterium isolated from a 20,000-to-30,000-year-old continuously frozen permafrost in the Kolyma region in Siberia. The survival strategies adopted to live at subzero temperatures include all the outer membrane molecules. A strategic involvement in the...

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Bibliographic Details
Published in:Extremophiles
Main Authors: A. Casillo, M. Ziaco, B. Lindner, E. Parrilli, D. Schwudke, A. Holgado, R. Beyaert, R. Lanzetta, M. L. Tutino, M. M. Corsaro
Other Authors: Casillo, A., Ziaco, M., Lindner, B., Parrilli, E., Schwudke, D., Holgado, A., Beyaert, R., Lanzetta, R., Tutino, M. L., Corsaro, M. M.
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
Lipid A
Mass spectrometry
Permafrost
Psychrobacter arcticus 273-4
Psychrotolerant
Microbiology
Molecular Medicine
permafrost
Siberia
Online Access:http://hdl.handle.net/11588/723934
https://doi.org/10.1007/s00792-018-1051-6
http://www.springerlink.com/app/home/journal.asp?wasp=a11a8a269a0040c1816cfffb60384506&referrer=parent&backto=linkingpublicationresults,1:100494,1
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Summary:Psychrobacter arcticus 273-4 is a Gram-negative bacterium isolated from a 20,000-to-30,000-year-old continuously frozen permafrost in the Kolyma region in Siberia. The survival strategies adopted to live at subzero temperatures include all the outer membrane molecules. A strategic involvement in the well-known enhancement of cellular membrane fluidity is attributable to the lipopolysaccharides (LPSs). These molecules covering about the 75% of cellular surface contribute to cold adaptation through structural modifications in their portions. In this work, we elucidated the exact structure of lipid A moiety obtained from the lipopolysaccharide of P. arcticus grown at 4 °C, to mimic the response to the real environment temperatures. The lipid A was obtained from the LPS by mild acid hydrolysis. The lipid A and its partially deacylated derivatives were exhaustively characterized by chemical analysis and by means of ESI Q-Orbitrap mass spectrometry. Moreover, biological assays indicated that P. arcticus 273-4 lipid A may behave as a weak TLR4 agonist.

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