Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H

Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered amodel to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34Hproduces two different extracellular polysaccharides, a capsular polysacchari...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek
Main Authors: Casillo, Angela, Ståhle, Jonas, PARRILLI, ERMENEGILDA, Sannino, Filomena, Mitchell, Daniel E., Pieretti, Giuseppina, Gibson, Matthew I., Marino, Gennaro, LANZETTA, ROSA, PARRILLI, MICHELANGELO, Widmalm, Göran, TUTINO, MARIA LUISA, CORSARO, MARIA MICHELA
Other Authors: Ståhle, Jona, Parrilli, Ermenegilda, Lanzetta, Rosa, Parrilli, Michelangelo, Tutino, MARIA LUISA, Corsaro, MARIA MICHELA
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Anti-freeze activity
Cold adaptation
Extracellular polysaccharide
NMR
Psychrophile
Microbiology
Molecular Biology
Arctic
Online Access:http://hdl.handle.net/11588/663533
https://doi.org/10.1007/s10482-017-0834-6
http://www.kluweronline.com/issn/0003-6072/
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Summary:Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered amodel to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34Hproduces two different extracellular polysaccharides, a capsular polysaccharide and amedium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and twodimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6- trideoxy-glucose (bacillosamine), and 2-acetamido-2- deoxyglucuronic acid with the following structure: ?4)-b-D-GlcpNAcA-(1 ?3)-b-D-QuipNAc4NAc- (1 ?3)-b-D-GalpNAc-(1 ?.The 3Dmodel, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 C, this molecule displays only a low ice recrystallization inhibition activity

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