Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study
X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an a...
| Main Authors: | , , , , , , , |
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| Other Authors: | , , , , , , , |
| Format: | Conference Object |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11588/518914 |
| _version_ | 1821606705646010368 |
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| author | MERLINO, ANTONELLO PICA, ANDREA VERGARA, ALESSANDRO M. R. Fuchs A. Balsamo F. Dworkowski G. Pompidor L. Mazzarella |
| author2 | Merlino, Antonello M. R., Fuch A., Balsamo Pica, Andrea F., Dworkowski G., Pompidor L., Mazzarella Vergara, Alessandro |
| author_facet | MERLINO, ANTONELLO PICA, ANDREA VERGARA, ALESSANDRO M. R. Fuchs A. Balsamo F. Dworkowski G. Pompidor L. Mazzarella |
| author_sort | MERLINO, ANTONELLO |
| collection | IRIS Università degli Studi di Napoli Federico II |
| description | X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an additional experimental source of denitrosylation. The system under investigation is the Hb from an Antarctic fish, Trematomus bernacchii (HbTb). To the best of our knowledge, this is the first Raman-assisted crystallographic evidence of X-ray induced NO-photodissociation at a 3rd generation synchrotron (beamline X10SA of the Swiss Light Source). |
| format | Conference Object |
| genre | Antarc* Antarctic |
| genre_facet | Antarc* Antarctic |
| geographic | Antarctic |
| geographic_facet | Antarctic |
| id | ftunivnapoliiris:oai:www.iris.unina.it:11588/518914 |
| institution | Open Polar |
| language | English |
| op_collection_id | ftunivnapoliiris |
| op_relation | ispartofbook:3rd Workshop on the Simultaneous Combination of Spectroscopies with X-ray Absorption, Scattering and Diffraction Techniques 3rd Workshop on the Simultaneous Combination of Spectroscopies with X-ray Absorption, Scattering and Diffraction Techniques firstpage:15 lastpage:15 http://hdl.handle.net/11588/518914 |
| publishDate | 2012 |
| record_format | openpolar |
| spelling | ftunivnapoliiris:oai:www.iris.unina.it:11588/518914 2025-01-16T19:09:06+00:00 Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study MERLINO, ANTONELLO PICA, ANDREA VERGARA, ALESSANDRO M. R. Fuchs A. Balsamo F. Dworkowski G. Pompidor L. Mazzarella Merlino, Antonello M. R., Fuch A., Balsamo Pica, Andrea F., Dworkowski G., Pompidor L., Mazzarella Vergara, Alessandro 2012 ELETTRONICO http://hdl.handle.net/11588/518914 eng eng ispartofbook:3rd Workshop on the Simultaneous Combination of Spectroscopies with X-ray Absorption, Scattering and Diffraction Techniques 3rd Workshop on the Simultaneous Combination of Spectroscopies with X-ray Absorption, Scattering and Diffraction Techniques firstpage:15 lastpage:15 http://hdl.handle.net/11588/518914 Raman X-ray crystallography photochemistry info:eu-repo/semantics/conferencePaper 2012 ftunivnapoliiris 2024-06-17T15:19:29Z X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an additional experimental source of denitrosylation. The system under investigation is the Hb from an Antarctic fish, Trematomus bernacchii (HbTb). To the best of our knowledge, this is the first Raman-assisted crystallographic evidence of X-ray induced NO-photodissociation at a 3rd generation synchrotron (beamline X10SA of the Swiss Light Source). Conference Object Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic |
| spellingShingle | Raman X-ray crystallography photochemistry MERLINO, ANTONELLO PICA, ANDREA VERGARA, ALESSANDRO M. R. Fuchs A. Balsamo F. Dworkowski G. Pompidor L. Mazzarella Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title | Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title_full | Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title_fullStr | Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title_full_unstemmed | Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title_short | Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study |
| title_sort | selective x-ray induced no-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted raman microscopy study |
| topic | Raman X-ray crystallography photochemistry |
| topic_facet | Raman X-ray crystallography photochemistry |
| url | http://hdl.handle.net/11588/518914 |