Selective X-ray induced NO-photodissociation in hemoglobin crystals: evidences from a crystallography-assisted Raman microscopy study

X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an a...

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Bibliographic Details
Main Authors: MERLINO, ANTONELLO, PICA, ANDREA, VERGARA, ALESSANDRO, M. R. Fuchs, A. Balsamo, F. Dworkowski, G. Pompidor, L. Mazzarella
Other Authors: Merlino, Antonello, M. R., Fuch, A., Balsamo, Pica, Andrea, F., Dworkowski, G., Pompidor, L., Mazzarella, Vergara, Alessandro
Format: Conference Object
Language:English
Published: 2012
Subjects:
Raman
X-ray crystallography
photochemistry
Antarctic
Antarc*
Online Access:http://hdl.handle.net/11588/518914
Description
Summary:X-ray induced radiation damage is a frequent phenomenon, especially when using third generation synchrotrons. Raman microscopy prior and after X-ray data collection can be a valuable tool to detect artifacts derived from radiation damage. Here we propose X-ray photolysis on crystals of NO-Hb as an additional experimental source of denitrosylation. The system under investigation is the Hb from an Antarctic fish, Trematomus bernacchii (HbTb). To the best of our knowledge, this is the first Raman-assisted crystallographic evidence of X-ray induced NO-photodissociation at a 3rd generation synchrotron (beamline X10SA of the Swiss Light Source).

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