Recombinant expression of Toluene o-Xylene Monooxygenase (ToMO) from Pseudomonas stutzeri OX1 in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.

The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125, isolated from Antarctic seawater, was used as recipient for a biodegradative gene of the mesophilic Pseudomonas stutzeri OX1. tou cluster, coding for Toluene-o-Xylene Monooxygenase (ToMO), was successfully cloned and expressed into a...

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Bibliographic Details
Main Authors: SIANI L., PAPA R., DI DONATO, ALBERTO, SANNIA, GIOVANNI
Other Authors: Siani, L., Papa, R., DI DONATO, Alberto, Sannia, Giovanni
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
Monooxygenase
Antarctic psychrophilic bacterium
Pseudomonas stutzeri OX1
Antarctic
Tac
Antarc*
Online Access:http://hdl.handle.net/11588/203934
Description
Summary:The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125, isolated from Antarctic seawater, was used as recipient for a biodegradative gene of the mesophilic Pseudomonas stutzeri OX1. tou cluster, coding for Toluene-o-Xylene Monooxygenase (ToMO), was successfully cloned and expressed into a “cold expression” vector. Catalytic parameters of the recombinant micro organisms on three different substrates were determined and compared with those exhibited by Escherichia coli recombinant cells expressing ToMO. Production of a catalytically efficient TAC/tou micro organism supports the possibility of developing specific degradative capabilities for the bioremediation of chemically contaminated marine environments and of industrial effluents characterised by low temperatures.

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