Solution structure of MT-nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps
The structure of [Cd-113(7)]-metallothionein (MT_nc)of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear H-1 NMR experiments and heteronuclear [H-1, Cd-113]-correlation spectroscopy. MT_nc is composed of an N-termi...
Published in: | Structure |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2003
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Subjects: | |
Online Access: | http://hdl.handle.net/11588/136605 https://doi.org/10.1016/S0969-2126(03)00044-3 |
Summary: | The structure of [Cd-113(7)]-metallothionein (MT_nc)of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear H-1 NMR experiments and heteronuclear [H-1, Cd-113]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with I I cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins. |
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