Solution structure of MT-nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps

The structure of [Cd-113(7)]-metallothionein (MT_nc)of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear H-1 NMR experiments and heteronuclear [H-1, Cd-113]-correlation spectroscopy. MT_nc is composed of an N-termi...

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Bibliographic Details
Published in:Structure
Main Authors: C. Capasso, V. Carginale, D. Di Maro, E. Parisi, CRESCENZI, ORLANDO, SPADACCINI, ROBERTA, TEMUSSI, PIERO ANDREA
Other Authors: C., Capasso, V., Carginale, Crescenzi, Orlando, D., Di Maro, E., Parisi, Spadaccini, Roberta, Temussi, PIERO ANDREA
Format: Article in Journal/Newspaper
Language:English
Published: 2003
Subjects:
Antarctic fish
electrostatic potential
ion exchange
NMR
poikilotherm organism
zinc homeostasis
Antarctic
The Antarctic
Antarc*
Online Access:http://hdl.handle.net/11588/136605
https://doi.org/10.1016/S0969-2126(03)00044-3
Description
Summary:The structure of [Cd-113(7)]-metallothionein (MT_nc)of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear H-1 NMR experiments and heteronuclear [H-1, Cd-113]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with I I cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.

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