DNA Repair in Deinococcus radiodurans

• Main Author: Timmins, Joanna
• Other Authors: Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Joseph Fourier, RUIGROK Rob
• Format: Doctoral or Postdoctoral Thesis
• Language: French
• Published: HAL CCSD 2012
• Subjects: cell imaging; structural genomic; Rec proteins; Imagerie cellulaire; génomique structurale; protéines Rec; [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Structural Biology [q-bio.BM]; Tromsø
• Online Access: https://hal.univ-grenoble-alpes.fr/tel-01301831; https://hal.univ-grenoble-alpes.fr/tel-01301831/document; https://hal.univ-grenoble-alpes.fr/tel-01301831/file/HDR%20Timmins.pdf

Deinococcus radiodurans is an unusual bacterium, displaying an outstanding resistance to desiccation, ionising radiation and DNA damaging agents. I became interested in this organism initially in the context of an ongoing structural genomics project at ESRF, which I joined in 2003. This structural genomics project led to the determination of a dozen crystal structures of proteins associated with the radiation-resistance phenotype of D. radiodurans. In addition, a number of complementary approaches were used to study this organism such as irradiation of cells on the medical beamline or coherent diffraction imaging of frozen cells. By 2006, I had become particularly involved in the structural and biochemical studies ofseveral essential DNA repair proteins involved in homologous recombination and the nucleotide-excision repair pathways. A strong collaboration with NorStruct in Tromsø also led me into the base-excision repair field. My interest in the DNA repair mechanisms of D. radiodurans brought me to the IBS in 2011 with an ATIP-Avenir grant, where I am now establishing my own team, focusing on the early steps of DNA repair, involving the specific recognition of DNA damages. My objectives are to determine the principles of damage localisation and recognition in cells by using high- and low-resolution structural studies combined with super-resolution imaging of the repair machinery in cells.