Arthrobacter ulcerisalmonis sp. nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Kämpfer, Peter, Busse, Hans-Jürgen, Schumann, Peter, Criscuolo, Alexis, Clermont, Dominique, Irgang, Rute, Poblete-Morales, Matías, Glaeser, Stefanie, Avendaño-Herrera, Ruben
• Other Authors: Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Veterinärmedizinische Universität Wien, Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH / Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Collection de l'Institut Pasteur (CIP), Institut Pasteur Paris (IP), Universidad Andrés Bello Santiago (UNAB), Interdisciplinary Center for Aquaculture Research = Centro Interdisciplinario de Investigación en Acuicultura Sustentable Concepción, Chile (INCAR), R. A.-H. would like to thank for financial support from grant FONDAP 15110027.
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Pseudarthrobacter; taxonomy; ulcerisalmonis; MESH: Animals; MESH: Aquaculture; MESH: Nucleic Acid Hybridization; MESH: Peptidoglycan; MESH: Phylogeny; MESH: RNA; Ribosomal; 16S; MESH: Salmo salar; MESH: Sequence Analysis; DNA; MESH: Ulcer; MESH: Vitamin K 2; MESH: Arthrobacter; MESH: Bacterial Typing Techniques; MESH: Base Composition; MESH: Cell Wall; MESH: Chile; MESH: DNA; Bacterial; MESH: Fatty Acids; MESH: Fish Diseases; [SDV]Life Sciences [q-bio]; [SDE]Environmental Sciences; Atlantic salmon; Salmo salar
• Online Access: https://hal-pasteur.archives-ouvertes.fr/pasteur-03259785; https://doi.org/10.1099/ijsem.0.004002

International audience A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon ( Salmo salar ), designated strain T11b T , was subjected to a comprehensive taxonomic investigation. A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %). The highest ANI value observed between the assembled genome of T11b T and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively. The major respiratory quinone was menaquinone MK-9(H 2 ). The polyamine pattern contained predominantly spermidine. The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride. Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol were also detected. The peptidoglycan was of the type A3α l -Lys– l -Ser –l -Thr– l -Ala (A11.23). In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C 15 : 0 , iso C 16 : 0 , anteiso C 17 : 0 ). Moderate to low DNA–DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11b T from the next related species. All these data point to the fact that strain T11b T represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp. nov. The type strain is T11b T (=CIP 111621 T =CCM 8854 T =LMG 30632 T =DSM 107127 T ).