ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations

International audience Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antivi...

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Bibliographic Details
Published in:mSphere
Main Authors: Rosani, Umberto, Bortoletto, Enrico, Montagnani, Caroline, Venier, Paola
Other Authors: Università degli Studi di Padova = University of Padua (Unipd), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2022
Subjects:
ADAR1
hyper-editing
oyster
OsHV-1
antiviral immunity
ADAR
RNA editing
bivalve
innate immunity
malacoherpesvirus
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Crassostrea gigas
Online Access:https://hal.archives-ouvertes.fr/hal-03636839
https://hal.archives-ouvertes.fr/hal-03636839/document
https://hal.archives-ouvertes.fr/hal-03636839/file/Rosani-2022-MSphere-ADAR.pdf
https://doi.org/10.1128/msphere.00011-22
Description
Summary:International audience Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq data sets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ∼0.5% of the oyster transcriptome and including several tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation, and epigenetic regulation that were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve “RNA editome,” available tools and technologies can be adapted to bivalve mollusks

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