Pesticide mixture toxicity assessment through in situ and laboratory approaches using embryo-larval stages of the pacific oyster (Magallana gigas)

• Published in: Marine Environmental Research
• Main Authors: Kuchovská, Eliška, Gonzalez, Patrice, Bláhová, Lucie, Barré, Mathilde, Gouffier, Corentin, Cachot, Jérôme, Roméro-Ramirez, Alicia, Bláha, Luděk, Morin, Bénédicte
• Other Authors: Masaryk University Brno (MUNI), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2021
• Subjects: Arcachon Bay; Coastal waters; Developmental abnormality; Gene expression; In situ; Mixtures; Pacific oyster; Pesticides; Sublethal effects; Swimming behavior; [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Pacific
• Online Access: https://hal.science/hal-03434109; https://hal.science/hal-03434109/document; https://hal.science/hal-03434109/file/Kuchovska%20et%20al.%20Marine%20environmental%20research.pdf; https://doi.org/10.1016/j.marenvres.2021.105390

International audience Worsened state of oysters in French Arcachon Bay, demand an investigation of possible causes. This study evaluated the effects of an environmentally relevant mixture of five common pesticides on the early-life stages of the Pacific oyster (Magallana gigas). Laboratory assays with artificial mixture and in situ transplantation were complementarily used to investigate a series of sublethal endpoints. The laboratory exposure revealed developmental toxicity at 0.32 μg/L, which corresponds to mixture concentrations in Arcachon Bay. Downregulation of some gene transcriptions was observed at environmental level. No difference in larvae development was revealed among the three sites in Arcachon Bay. This study was the first to evaluate locomotion of oyster larvae exposed in situ. Suspected poor water quality in the inner part of Arcachon Bay was reflected by impairment at the molecular level. In conclusion, current concentrations of the tested pesticides in Arcachon Bay hinder larval development and affect several biological functions.