Proposal of 'Candidatus Frankia alpina', the uncultured symbiont of Alnus alnobetula and A. incana that forms spore-containing nitrogen-fixing root nodules

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Pozzi, Adrien, Herrera-Belaroussi, Aude, Schwob, Guillaume, Bautista-Guerrero, Hector, Bethencourt, Lorine, Fournier, Pascale, Dubost, Audrey, Abrouk, Danis, Normand, Philippe, Fernandez, Maria
• Other Authors: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lyon
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: alder; spores; Symbiotic. Abbreviations: ANI; average nucleotide identity; ML; maximum-likelihood; NJ; neighbour-joining; [SDV]Life Sciences [q-bio]; Subarctic
• Online Access: https://hal.science/hal-03378158; https://hal.science/hal-03378158/document; https://hal.science/hal-03378158/file/2020%20Pozzi%20alpina%205453_ijsem004433.pdf; https://doi.org/10.1099/ijsem.0.004433

International audience The members of the genus Frankia are, with a few exceptions, a group of nitrogen-fixing symbiotic actinobacteria that nodulate mostly woody dicotyledonous plants belonging to three orders, eight families and 23 genera of pioneer dicots. These bacteria have been characterized phylogenetically and grouped into four molecular clusters. One of the clusters, cluster 1 contains strains that induce nodules on Alnus spp. ( Betulaceae ), Myrica spp., Morella spp. and Comptonia spp. ( Myricaceae ) that have global distributions. Some of these strains produce not only hyphae and vesicles, as other cluster 1 strains do, but also numerous sporangia in their host symbiotic tissues, hence their phenotype being described as spore-positive (Sp+). While Sp+ strains have resisted repeated attempts at cultivation, their genomes have recently been characterized and found to be different from those of all described species, being markedly smaller than their phylogenetic neighbours. We thus hereby propose to create a ' Candidatus Frankia alpina' species for some strains present in nodules of Alnus alnobetula and A. incana that grow in alpine environments at high altitudes or in subarctic environments at high latitudes.