Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line
International audience Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://hal.inrae.fr/hal-03318569 https://doi.org/10.1016/j.dci.2020.103716 |
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ftunivnantes:oai:HAL:hal-03318569v1 2023-05-15T15:28:16+02:00 Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line Andresen, Adriana Magalhães Santos Boudinot, Pierre Gjøen, Tor University of Oslo (UiO) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Department of Pharmacy, Universityof Oslo general faculty grant number # 103806 INRAE ANR-16-CE20-0002,Fish-RNAvax,Vaccins ARN éco-compatibles pour l'induction de réponses immunitaires protectrices chez le poisson d'élevage(2016) 2020-09 https://hal.inrae.fr/hal-03318569 https://doi.org/10.1016/j.dci.2020.103716 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.dci.2020.103716 info:eu-repo/semantics/altIdentifier/pmid/32360383 hal-03318569 https://hal.inrae.fr/hal-03318569 doi:10.1016/j.dci.2020.103716 PUBMED: 32360383 WOS: 000539156400009 ISSN: 0145-305X Developmental and Comparative Immunology https://hal.inrae.fr/hal-03318569 Developmental and Comparative Immunology, 2020, 110, ⟨10.1016/j.dci.2020.103716⟩ https://www.sciencedirect.com/journal/developmental-and-comparative-immunology Atlantic salmon Adjuvant Transcriptome Poly (I:C) VaccineISAV [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology info:eu-repo/semantics/article Journal articles 2020 ftunivnantes https://doi.org/10.1016/j.dci.2020.103716 2023-03-08T02:49:38Z International audience Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of an Atlantic salmon cell line to compare innate immune responses against ISAV and an experimental viral vaccine adjuvant: poly (I:C). Induction of interferon and interferon induced genes were observed after both treatments, but often with different amplitude and kinetics. Using KEGG ortholog database and available software from Bioconductor we could specify a complete bioinformatic pipeline for analysis of transcriptomic data from Atlantic salmon, a feature not previously available. We have identified important differences in the transcriptional profile of Atlantic salmon cells exposed to viral infection and a viral vaccine adjuvant candidate, poly (I:C). This report increases our knowledge of viral host-pathogen interaction in salmon and to which extent these can be mimicked by adjuvant compounds. Article in Journal/Newspaper Atlantic salmon Université de Nantes: HAL-UNIV-NANTES Developmental & Comparative Immunology 110 103716 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Atlantic salmon Adjuvant Transcriptome Poly (I:C) VaccineISAV [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
spellingShingle |
Atlantic salmon Adjuvant Transcriptome Poly (I:C) VaccineISAV [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Andresen, Adriana Magalhães Santos Boudinot, Pierre Gjøen, Tor Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
topic_facet |
Atlantic salmon Adjuvant Transcriptome Poly (I:C) VaccineISAV [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
description |
International audience Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of an Atlantic salmon cell line to compare innate immune responses against ISAV and an experimental viral vaccine adjuvant: poly (I:C). Induction of interferon and interferon induced genes were observed after both treatments, but often with different amplitude and kinetics. Using KEGG ortholog database and available software from Bioconductor we could specify a complete bioinformatic pipeline for analysis of transcriptomic data from Atlantic salmon, a feature not previously available. We have identified important differences in the transcriptional profile of Atlantic salmon cells exposed to viral infection and a viral vaccine adjuvant candidate, poly (I:C). This report increases our knowledge of viral host-pathogen interaction in salmon and to which extent these can be mimicked by adjuvant compounds. |
author2 |
University of Oslo (UiO) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Department of Pharmacy, Universityof Oslo general faculty grant number # 103806 INRAE ANR-16-CE20-0002,Fish-RNAvax,Vaccins ARN éco-compatibles pour l'induction de réponses immunitaires protectrices chez le poisson d'élevage(2016) |
format |
Article in Journal/Newspaper |
author |
Andresen, Adriana Magalhães Santos Boudinot, Pierre Gjøen, Tor |
author_facet |
Andresen, Adriana Magalhães Santos Boudinot, Pierre Gjøen, Tor |
author_sort |
Andresen, Adriana Magalhães Santos |
title |
Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
title_short |
Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
title_full |
Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
title_fullStr |
Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
title_full_unstemmed |
Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line |
title_sort |
kinetics of transcriptional response against poly (i:c) and infectious salmon anemia virus (isav) in atlantic salmon kidney (ask) cell line |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.inrae.fr/hal-03318569 https://doi.org/10.1016/j.dci.2020.103716 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 0145-305X Developmental and Comparative Immunology https://hal.inrae.fr/hal-03318569 Developmental and Comparative Immunology, 2020, 110, ⟨10.1016/j.dci.2020.103716⟩ https://www.sciencedirect.com/journal/developmental-and-comparative-immunology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.dci.2020.103716 info:eu-repo/semantics/altIdentifier/pmid/32360383 hal-03318569 https://hal.inrae.fr/hal-03318569 doi:10.1016/j.dci.2020.103716 PUBMED: 32360383 WOS: 000539156400009 |
op_doi |
https://doi.org/10.1016/j.dci.2020.103716 |
container_title |
Developmental & Comparative Immunology |
container_volume |
110 |
container_start_page |
103716 |
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1766358641728815104 |