Kinetics of transcriptional response against poly (I:C) and infectious salmon anemia virus (ISAV) in Atlantic salmon kidney (ASK) cell line

International audience Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of...

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Bibliographic Details
Published in:Developmental & Comparative Immunology
Main Authors: Andresen, Adriana Magalhães Santos, Boudinot, Pierre, Gjøen, Tor
Other Authors: University of Oslo (UiO), Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Pharmacy, Universityof Oslo general faculty grant number # 103806, INRAE, ANR-16-CE20-0002,Fish-RNAvax,Vaccins ARN éco-compatibles pour l'induction de réponses immunitaires protectrices chez le poisson d'élevage(2016)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2020
Subjects:
Atlantic salmon
Adjuvant
Transcriptome
Poly (I:C)
VaccineISAV
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Online Access:https://hal.inrae.fr/hal-03318569
https://doi.org/10.1016/j.dci.2020.103716
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Summary:International audience Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of an Atlantic salmon cell line to compare innate immune responses against ISAV and an experimental viral vaccine adjuvant: poly (I:C). Induction of interferon and interferon induced genes were observed after both treatments, but often with different amplitude and kinetics. Using KEGG ortholog database and available software from Bioconductor we could specify a complete bioinformatic pipeline for analysis of transcriptomic data from Atlantic salmon, a feature not previously available. We have identified important differences in the transcriptional profile of Atlantic salmon cells exposed to viral infection and a viral vaccine adjuvant candidate, poly (I:C). This report increases our knowledge of viral host-pathogen interaction in salmon and to which extent these can be mimicked by adjuvant compounds.

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