Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca

International audience The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 f...

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Bibliographic Details
Published in:Reproduction, Fertility and Development
Main Authors: Zarski, Daniel, Krejszeff, Slawomir, Palinska, Katarzyna, Targonska, Katarzyna, Kupren, Krzysztof, Fontaine, Pascal, Kestemont, Patrick, Kucharczyk, Dariusz
Other Authors: University of Warmia and Mazury Olsztyn, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Université de Namur Namur (UNamur)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2012
Subjects:
egg activation
gametes
ONCORHYNCHUS-MYKISS
LARVAL DEVELOPMENT
CYPRINFISH
RAINBOW-TROUT
ACTIVATION
artificial insemination
fertilisation
PERCH PERCA-FLUVIATILIS
GADUS-MORHUA L
EURASIAN PERCH
LIPDROPLETS
L
[SDV]Life Sciences [q-bio]
[INFO]Computer Science [cs]
Gadus morhua
Online Access:https://hal.inrae.fr/hal-02646319
https://doi.org/10.1071/RD11264
Description
Summary:International audience The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16 degrees C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5 min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5 min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.

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