Comparing single-feeding and multi-feeding approach for experimentally assessing trophic transfer of metals in fish

• Published in: Environmental Toxicology and Chemistry
• Main Authors: Pouil, Simon, Warnau, Michel, Oberhänsli, François, Teyssié, Jean-Louis, Bustamante, Paco, Metian, Marc
• Other Authors: Environment Laboratories (IAEA), International Atomic Energy Agency Vienna (IAEA), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2017
• Subjects: Fish; Metals; Radioecology; Trophic transfer; Assimilation efficiency; [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology; Scophthalmus maximus; Turbot
• Online Access: https://hal.science/hal-01694264; https://hal.science/hal-01694264/document; https://hal.science/hal-01694264/file/Pouil%20et%20al%202017%20ETC.pdf; https://doi.org/10.1002/etc.3646

International audience Diet is an important pathway for metal uptake in marine organisms, and assimilation efficiency (AE) is one of the most relevant parameter to quantify trophic transfer of metals along aquatic food webs. The most commonly used method to estimate this parameter is pulse-chase feeding using radiolabeled food. This approach is, however, based on several assumptions that are not always tested in experimental context. The present work aimed at validating the approach by assessing single-feeding and multiple-feeding approaches, using a model species (the turbot Scophthalmus maximus). Using the kinetic data obtained from the single-feeding experiment, we tested whether the reconstruction of a multi-feeding was consistent with data provided by an actual multi-feeding performed under the same experimental conditions. Our results validated the single-feeding approach.