First genetic linkage map for comparative mapping and QTL screening of brill (Scophthalmus rhombus)

• Published in: Aquaculture
• Main Authors: Hermida, Miguel, Rodríguez-Ramilo, Silvia Teresa, Hachero-Cruzado, Ismael, Herrera, Marcelino, Sciara, Andrés A., Bouza, Carmen, Fernández, Jesús, Martínez, Paulino
• Other Authors: Universidade de Santiago de Compostela Spain (USC ), Dept Bioquim Genet & Inmunol, Universidade de Vigo, Dept Mejora Genet Anim, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Inst Invest & Formac Agr & Pesquera Andalucia IFA, Centre Agua Pino
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: brill; Scophthalmus rhombus; turbot; cross-specie microsatellite; genetic map; growth-related qtl; [SDV]Life Sciences [q-bio]; Turbot
• Online Access: https://hal.archives-ouvertes.fr/hal-01448410; https://doi.org/10.1016/j.aquaculture.2013.02.041

Genetic maps constitute valuable tools to detect genomic regions associated with complex traits and to go forward to understand their genetic basis. Flatfish include several species of great commercial value for which increasing genomic resources are available including genetic maps and EST databases. Application of comparative mapping strategies to flatfish is relevant to obtain genetic information associated with productive traits. The brill (Scophthalmus rhombus) is a flatfish species closely related to turbot (S. maximus) whose meat is highly appreciated in the Spanish market. The Junta de Andalucia local Government has begun a program to adapt this species to captivity for its future production. In this study, we developed the first genetic map of brill using the current turbot genetic map as starting point. This strategy enabled us to select a number of homogeneously distributed markers in the turbot map and to apply cross-species microsatellite amplification to obtain informative markers. Nearly two hundred microsatellites from the framework turbot map were used for validation, and 100 markers were finally informative for mapping. The parents and offspring of the two families (54 and 88, respectively) used to construct the genetic map were genotyped with this panel. All markers, except eleven, were successfully grouped and ordered in 24 linkage groups. Linkage groups and order of markers were highly consistent with the previous turbot genetic map. Linkage map information was used to carry out a preliminary study on growth-related QTL for body weight, length and Fulton's condition factor in the two families, as the main phenotypic traits of interest in this species. (C) 2013 Elsevier B.V. All rights reserved.