Genetic diversity of the golden potato cyst nematode Globodera rostochiensis and determination of the origin of populations in Quebec, Canada

• Published in: Molecular Phylogenetics and Evolution
• Main Authors: Boucher, Annie Christine, Mimee, Benjamin, Montarry, Josselin, Bardou-Valette, Sylvie, Belair, Guy, Moffett, Peter, Grenier, Eric
• Other Authors: Agriculture and Agri-Food (AAFC), Département de biologie Sherbrooke (UdeS), Faculté des sciences Sherbrooke (UdeS), Université de Sherbrooke (UdeS)-Université de Sherbrooke (UdeS), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1)-AGROCAMPUS OUEST, Centre de Recherche Les Buissons Inc. through Agriculture and Agri-food Canada Growing Canadian Agri-Innovations Program; Institut National de la Recherche Agronomique; Fonds de Recherche Quebecois Nature et Technologie; Centre SEVE; National Sciences and Engineering Research Council
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2013
• Subjects: MULTILOCUS GENOTYPE DATA; MICROSATELLITE MARKERS; EUROPEAN POPULATIONS; INDIVIDUALS; DIFFERENTIATION; LOCI; PALLIDA; INFERENCE; PROGRAM; NUMBER; Globodera rostochiensis; Microsatellite; Population genetics; Multiple introductions; Genetic structure; Potato cyst nematode; [SDV]Life Sciences [q-bio]; British Columbia; Canada; Newfoundland
• Online Access: https://hal.science/hal-01208645; https://doi.org/10.1016/j.ympev.2013.05.020

International audience The golden cyst nematode (Globodera rostochiensis), native to South America, has been introduced in many parts of the world, including Europe and North America. Recently, it was found for the first time in the province of Quebec, Canada in the locality of St. Amable near Montreal. To date, very few studies have examined the population genetics of this pest. Consequently, there is a lack of knowledge about the genetic structure and evolution of this nematode. In this study, twelve new microsatellite markers were developed in order to explore these questions. These markers were used to genotype fifteen populations originating from different regions of the world, including five from Canada. Within populations, the highest genetic diversity was consistently observed in the populations from Bolivia, the postulated region of origin of the golden nematode, and the lowest in populations from British Columbia (Canada) and New York (USA). The two Quebec populations were very similar to each other and to the population found in Newfoundland, but surprisingly, they were significantly different from three other North American populations including those from New York and British Columbia. Based on our results, we conclude that the golden cyst nematode has been introduced in North America at least twice from distinct regions of the world. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.