Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization

• Published in: Biotechnology Journal
• Main Authors: Pavlidis, Ioannis V, Tzafestas, Kyriakos, Stamatis, Haralambos
• Other Authors: Biological Applications and Technologies, University of Ioannina
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2010
• Subjects: Life Sciences; Antarc*; Antarctica
• Online Access: https://hal.archives-ouvertes.fr/hal-00552348; https://hal.archives-ouvertes.fr/hal-00552348/document; https://hal.archives-ouvertes.fr/hal-00552348/file/PEER_stage2_10.1002%252Fbiot.201000052.pdf; https://doi.org/10.1002/biot.201000052

International audience The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ionic liquids, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70 o C, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behaviour observed.