Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization
International audience The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used a...
| Published in: | Biotechnology Journal |
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| Main Authors: | , , |
| Other Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
HAL CCSD
2010
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| Subjects: | |
| Online Access: | https://hal.archives-ouvertes.fr/hal-00552348 https://hal.archives-ouvertes.fr/hal-00552348/document https://hal.archives-ouvertes.fr/hal-00552348/file/PEER_stage2_10.1002%252Fbiot.201000052.pdf https://doi.org/10.1002/biot.201000052 |
| Summary: | International audience The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ionic liquids, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70 o C, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behaviour observed. |
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