| Summary: | Role of biosurfactants has shown renewed interest; hence, search for readily available natural resources of these materials is continuing. Chicken viscera and bahera oil were used as natural sources of oleic acid and its methyl ester, respectively, for the preparation of biosurfactants. Enzymatic esterification and transesterification of oleic acid were performed with sorbitol, fructose and ascorbic acid using Candida antarctica lipase, and their isolated yields were compared. Oleyl esters of sorbitol, fructose and ascorbic acid were produced 27, 21 and 12%, respectively. Transesterification reaction gave better yield than esterification reaction. Synthesized products were confirmed by nuclear magnetic resonance spectroscopy. Radical scavenging activity of synthesized ascorbyl oleate was compared with ascorbic acid using 1,1-diphenyl-2-picryl hydrazyl radical. Ascorbyl oleate showed better antioxidant activity than ascorbic acid.
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