| Summary: | Instituto de Investigaciones Agropecuarias y Forestales. Facultad de Biología. Facultad de Medicina Veterinaria y Zootecnia. Facultad de Agrobiología. Facultad de Químico Farmacobiología. Programa Institucional de Maestría en Ciencias Biológicas The Canidae are a family of mammals that include dogs, wolves, coyotes and others, including 35 species (spp) worldwide. Mexico has 4 spp, of which the Coyote (Canis latrans) is one of these species that is highly related to the wolf (Canis lupus), being the species with the greatest distribution in America, from Alaska to Panama. In the Mexican Republic, 10 subspecies of the 19 existing in the world have been reported; however, no molecular studies have yet been done to confirm this. Taxonomically, this classification is discarded due to the interbreeding and location of individuals because it is a very prolific species. The use of molecular markers such as Cytochrome Oxidase I (COI) and Control Region (CR) of mitochondrial DNA (mtDNA) allows us to identify and characterize species. In this work, the genetic diversity and phylogenetic relationships of coyotes were analyzed by using mtDNA molecular markers [Cytochrome oxidase I (COI), Control Region (CR)], and nuclear DNA (DRB1 and ZDHHC16) since are the most reported for the species. The objective was to analyze the genetic diversity and phylogeny of the Canis latrans species present in Mexican zoos, obtained from blood samples of 23 individuals. A high genetic diversity was identified, finding that coyotes from Mexico have a high nucleotide and haplotype diversity for the COI, D-loop and DRB1 genes, in addition to finding new haplotypes for C. latrans in the four genes studied, identified in the phylogenetic analyses. This work represents a useful strategy for the investigation of understudied species, which nevertheless have to be in some state of threatened or endangered. Los cánidos (Canidae) son una familia de mamíferos donde están agrupados los perros, lobos, coyotes y afines, incluyendo 35 especies (spp) en ...
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