| Summary: | Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, -lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. This work was supported by Discovery (A.K., W.G.D.F.) and CreateH2O (A.F.) grants funded by the Natural Science and Engineering Research Council of Canada as well as a University of Manitoba Start-up grant (E.K.). D.M.F. was funded by a Canada Research Chair grant to Peter Loewen, Department of Microbiology, University of Manitoba. R.P. is funded by a postdoctoral fellowship from the Government of Madhya Pradesh, India.
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