Molecular evolution of Trichuris muris isolated from different Muridae hosts in Europe

A phylogeographic study was carried out of Trichuris muris, nematode parasitizing Murinae rodents from the Muridae family, isolated from four different hosts and from different geographical regions of Europe by amplification and sequencing of the ITS1-5.8S-ITS2 fragment of the ribosomal DNA. T. muri...

Full description

Bibliographic Details
Published in:Parasitology Research
Main Authors: Callejón, Rocio, de Rojas, Manuel, Nieberding, Caroline M., Foronda, Pilar, Feliú, Carlos, Guevara, Diego, Cutillas, Cristina
Other Authors: Faculty of Pharmacy, University of Seville - Department of Microbiology and Parasitology, UCL - SST/ELI/ELIB - Biodiversity, La Laguna University,Tenerife, Spain - Canaries Tropical Disease and Public Health Institute, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain - Department of Microbiology and Parasitology
Format: Article in Journal/Newspaper
Language:English
Published: Springer 2010
Subjects:
Animals
DNA
Ribosomal Spacer - analysis
Trichuriasis - parasitology
veterinary
Trichuris - genetics
isolation & purification
physiology
Europe
Evolution
Molecular
Host-Parasite Interactions
Muridae - classification
parasitology
Phylogeny
Polymorphism
Genetic
RNA
Ribosomal
5.8S - genetics
Rodent Diseases - parasitology
3123
QH301
Rattus rattus
Online Access:http://hdl.handle.net/2078.1/104158
https://doi.org/10.1007/s00436-010-1908-9
Description
Summary:A phylogeographic study was carried out of Trichuris muris, nematode parasitizing Murinae rodents from the Muridae family, isolated from four different hosts and from different geographical regions of Europe by amplification and sequencing of the ITS1-5.8S-ITS2 fragment of the ribosomal DNA. T. muris was found in the Apodemus sylvaticus, Apodemus flavicollis, Mus domesticus, and Rattus rattus rodents. The molecular results confirm the presence of DNA polymorphisms among T. muris isolates from Europe. The present study shows two clear-cut geographical and genetic lineages: one of them is widespread from northern Spain (Catalonia) to Denmark (Western European region), while the second is widespread in the Eastern European region (Croatia, Rumania, and Turkey). These two genotypes can be easily distinguished by a PCR-RFLP analysis of this sequence with the ApalI restriction enzyme. Moreover, networks and phylogenetic reconstructions also reveal that T. muris from various Murinae rodents did not differentiate according to the host species that they parasitize. Furthermore, T. muris isolated from The Canary Islands revealed a typical haplotype (H6) only present in The Canary Islands and not in continental Europe. It is suggested that one haplotype from La Gomera Island is the ancestor of T. muris in the Canary Islands

Similar Items