Comparison of mesoporous silicate supports for the immobilisation and activity of cytochrome c and lipase

peer-reviewed The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised on to, SBA-15 and a porous spherical silicate material (PPS), were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB e...

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Bibliographic Details
Published in:Journal of Molecular Catalysis B: Enzymatic
Main Authors: Abdallah, Noreldeen Hassan., Schlumpberger, Miriam., Gaffney, Darragh, Hanrahan, John P., Tobin, Joseph M., Magner, Edmond
Other Authors: IRC, SFI, HEA
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2014
Subjects:
mesoporous silicate
immobilisation
lipase
cytochrome c
antartic*
Online Access:http://hdl.handle.net/10344/4698
https://doi.org/10.1016/j.molcatb.2014.06.007
Description
Summary:peer-reviewed The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised on to, SBA-15 and a porous spherical silicate material (PPS), were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited higher catalytic activity and stability on SBA-15 when compared to PPS, while cytochrome c showed similar catalytic activity on both materials. The activity of CALB immobilised on SBA-15 was retained (95%) after 7 uses, while CALB immobilised on PPS retained only 43% activity. Such changes can be mainly ascribed to the different physical properties (pore volume, surface area and pore shape) of the supports.

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