Comparison of mesoporous silicate supports for the immobilisation and activity of cytochrome c and lipase

The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised on to, SBA-15 and a porous spherical silicate material (PPS), were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited highe...

Full description

Bibliographic Details
Main Authors: Noreldeen Hassan. Abdallah, Miriam. Schlumpberger, Darragh Gaffney, John P. Hanrahan, Joseph M. Tobin, EDMOND MAGNER
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2014
Subjects:
mesoporous silicate
immobilisation
lipase
cytochrome c
antartic*
Online Access:https://figshare.com/articles/journal_contribution/Comparison_of_mesoporous_silicate_supports_for_the_immobilisation_and_activity_of_cytochrome_c_and_lipase/19815067
Description
Summary:The activity and stability of Candida antartica lipase B (CALB) and cytochrome c immobilised on to, SBA-15 and a porous spherical silicate material (PPS), were examined. The materials possess similar pore diameters but have different morphologies, pore volumes and surface areas. CALB exhibited higher catalytic activity and stability on SBA-15 when compared to PPS, while cytochrome c showed similar catalytic activity on both materials. The activity of CALB immobilised on SBA-15 was retained (95%) after 7 uses, while CALB immobilised on PPS retained only 43% activity. Such changes can be mainly ascribed to the different physical properties (pore volume, surface area and pore shape) of the supports.

Similar Items