Discriminating glacial races of burbot (Lota lota) by means of PCR-RF-SSCP (PRS) analysis of the mitochondrial control region

• Main Authors: Van Houdt, Jkj, De Cleyn, L, Perretti, A, Volckaert, Filip
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2006
• Subjects: accuracy; diversity; endonuclease; screening; sensitivity; SSCP; Strand Conformation Polymorphism; Polymerase Chain-Reaction; Point Mutations; Rapid Method; Dna; Burbot; Lota lota; lota
• Online Access: https://lirias.kuleuven.be/handle/123456789/182253

We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR-RF-SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations did not result in a detectable change that resulted in 11 pairwise comparisons (2%) with indistinguishable haplotypes. The haplotypes that could not be discriminated were always characteristic for a certain assemblage (glacial race). As such, this method provides a reliable alternative to direct sequencing for phylogeographic studies in case intrapopulation diversity is limited. status: published