Discriminating glacial races of burbot (Lota lota) by means of PCR-RF-SSCP (PRS) analysis of the mitochondrial control region

We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR-RF-SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations...

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Bibliographic Details
Main Authors: Van Houdt, Jkj, De Cleyn, L, Perretti, A, Volckaert, Filip
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
accuracy
diversity
endonuclease
screening
sensitivity
SSCP
Strand Conformation Polymorphism
Polymerase Chain-Reaction
Point Mutations
Rapid Method
Dna
Burbot
Lota lota
lota
Online Access:https://lirias.kuleuven.be/handle/123456789/182253
Description
Summary:We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR-RF-SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations did not result in a detectable change that resulted in 11 pairwise comparisons (2%) with indistinguishable haplotypes. The haplotypes that could not be discriminated were always characteristic for a certain assemblage (glacial race). As such, this method provides a reliable alternative to direct sequencing for phylogeographic studies in case intrapopulation diversity is limited. status: published

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