Discriminating glacial races of burbot (Lota lota) by means of PCR-RF-SSCP (PRS) analysis of the mitochondrial control region
We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR-RF-SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations...
Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2006
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Subjects: | |
Online Access: | https://lirias.kuleuven.be/handle/123456789/182253 |
Summary: | We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR-RF-SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations did not result in a detectable change that resulted in 11 pairwise comparisons (2%) with indistinguishable haplotypes. The haplotypes that could not be discriminated were always characteristic for a certain assemblage (glacial race). As such, this method provides a reliable alternative to direct sequencing for phylogeographic studies in case intrapopulation diversity is limited. status: published |
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