Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa
P. 1119-1128 Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologi...
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ftunivleon:oai:buleria.unileon.es:10612/10673 2023-05-15T18:42:10+02:00 Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa Nicolás, M. Álvarez García, Mercedes Barragán Santos, Santiago Martínez Pastor, Felipe Chamorro Álvarez, César Ángel Álvarez Rodríguez, Manuel Paz Cabello, Paulino de Anel Rodríguez, Luis Biologia Celular Facultad de Ciencias Biologicas y Ambientales 2012-04-01 http://hdl.handle.net/10612/10673 https://www.sciencedirect.com/science/article/pii/S0093691X11005310 eng eng Elsevier Theriogenology, 2012, vol. 77, n. 6 https://www.sciencedirect.com/science/article/pii/S0093691X11005310 http://hdl.handle.net/10612/10673 Veterinaria Centrifugation Density gradient Cryopreservation Brown bear Spermatozoa info:eu-repo/semantics/article 2012 ftunivleon 2022-12-26T13:26:13Z P. 1119-1128 Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample. SI Article in Journal/Newspaper Ursus arctos Universidad de León: BULERIA |
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Open Polar |
collection |
Universidad de León: BULERIA |
op_collection_id |
ftunivleon |
language |
English |
topic |
Veterinaria Centrifugation Density gradient Cryopreservation Brown bear Spermatozoa |
spellingShingle |
Veterinaria Centrifugation Density gradient Cryopreservation Brown bear Spermatozoa Nicolás, M. Álvarez García, Mercedes Barragán Santos, Santiago Martínez Pastor, Felipe Chamorro Álvarez, César Ángel Álvarez Rodríguez, Manuel Paz Cabello, Paulino de Anel Rodríguez, Luis Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
topic_facet |
Veterinaria Centrifugation Density gradient Cryopreservation Brown bear Spermatozoa |
description |
P. 1119-1128 Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample. SI |
author2 |
Biologia Celular Facultad de Ciencias Biologicas y Ambientales |
format |
Article in Journal/Newspaper |
author |
Nicolás, M. Álvarez García, Mercedes Barragán Santos, Santiago Martínez Pastor, Felipe Chamorro Álvarez, César Ángel Álvarez Rodríguez, Manuel Paz Cabello, Paulino de Anel Rodríguez, Luis |
author_facet |
Nicolás, M. Álvarez García, Mercedes Barragán Santos, Santiago Martínez Pastor, Felipe Chamorro Álvarez, César Ángel Álvarez Rodríguez, Manuel Paz Cabello, Paulino de Anel Rodríguez, Luis |
author_sort |
Nicolás, M. |
title |
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
title_short |
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
title_full |
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
title_fullStr |
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
title_full_unstemmed |
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
title_sort |
evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (maxifreeze, cushion fluid equine, and puresperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/10612/10673 https://www.sciencedirect.com/science/article/pii/S0093691X11005310 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
Theriogenology, 2012, vol. 77, n. 6 https://www.sciencedirect.com/science/article/pii/S0093691X11005310 http://hdl.handle.net/10612/10673 |
_version_ |
1766231782002262016 |