Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa

P. 1119-1128 Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologi...

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Bibliographic Details
Main Authors: Nicolás, M., Álvarez García, Mercedes, Barragán Santos, Santiago, Martínez Pastor, Felipe, Chamorro Álvarez, César Ángel, Álvarez Rodríguez, Manuel, Paz Cabello, Paulino de, Anel Rodríguez, Luis
Other Authors: Biologia Celular, Facultad de Ciencias Biologicas y Ambientales
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2012
Subjects:
Veterinaria
Centrifugation
Density gradient
Cryopreservation
Brown bear
Spermatozoa
Ursus arctos
Online Access:http://hdl.handle.net/10612/10673
https://www.sciencedirect.com/science/article/pii/S0093691X11005310
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Summary:P. 1119-1128 Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample. SI

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