Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge

Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identi...

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Bibliographic Details
Published in:Bioresource Technology
Main Authors: Beauchamp, Chantal Jeanne, Lévesque, Gabriel, Chalifour, François-Philippe, Prévost, Danielle
Format: Other/Unknown Material
Language:English
Published: Elsevier 2016
Subjects:
Azote > Fixation
Compost
Glucose
Pseudomonas
Pâte à papier > Industrie > Sous-produits
Produits de la pâte à papier
Bois > Déchets > Recyclage
Arctic
Online Access:https://hdl.handle.net/20.500.11794/350
https://doi.org/10.1016/j.biortech.2005.04.041
Description
Summary:Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 degrees C, both isolates revealed that N2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 degrees C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source. Keywords: Dinitrogen-fixing bacteria; Compost; Paper sludge; Glucose; Pseudomonas balearica; Pseudomonas putida

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