Cross-reactivity of Anti-human, Anti-porcine and Anti-bovine Cytokine Antibodies with Cetacean Tissues

The cross-reactivity of monoclonal antibodies specific for human, porcine and bovine cytokines was evaluated for three cetacean species: Atlantic spotted dolphins (Stenella frontalis), striped dolphins (Stenella coeruleoalba) and fin whales (Balaenoptera physalus). Formalin-fixed and snap-frozen tis...

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Bibliographic Details
Published in:Journal of Comparative Pathology
Main Authors: Jáber Mohamad, José Raduán, Pérez, J., Zafra, R., Herráez Thomas, Pedro Manuel, Rodríguez Guisado, Francisco, Arbelo Hernández, Manuel Antonio, Espinosa de los Monteros y Zayas, Antonio, Fernández Rodríguez, Antonio Jesús
Other Authors: Arbelo, Manuel, Perez, Jose, Fernandez, Antonio, Zafra Leva, Rafael, 7004667021, 55597088334, 23029634000, 57194503614, 21641919200, 6603561403, 36928058400, 56673009900, 959492, 319037, 770447, 523902, 6971818, 667707, 700159, 176520
Language:English
Published: 0021-9975 2010
Subjects:
310907 Patología
Cetacean
Cross reactivity
Cytokine
Immunohistochemistry
Balaenoptera physalus
Online Access:http://hdl.handle.net/10553/43361
https://doi.org/10.1016/j.jcpa.2010.01.001
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Summary:The cross-reactivity of monoclonal antibodies specific for human, porcine and bovine cytokines was evaluated for three cetacean species: Atlantic spotted dolphins (Stenella frontalis), striped dolphins (Stenella coeruleoalba) and fin whales (Balaenoptera physalus). Formalin-fixed and snap-frozen tissue sections of lung, spleen, liver and mesenteric lymph node were evaluated. T and B lymphocytes and monocytes/macrophages were detected by use of anti-human CD3, IgG and lysozyme polyclonal antibodies (pAbs), respectively. These reagents were successfully applied to both fixed and frozen tissues. Anti-human interleukin (IL)-1α, IL-1β, IL-8, tumour necrosis factor (TNF)-α and CD25, anti-porcine IL-2, IL-6, IL-10, and anti-bovine IL-4 and interferon (IFN)-γ antibodies produced immunolabelling in cetacean snap-frozen lymph node sections similar to that obtained with tissue from the species of origin, but they did not react with formalin-fixed tissue sections. Anti-porcine IL-12 pAb did not react with snap-frozen cetacean tissue samples. Macrophages and lymphocytes were the most common cells immunolabelled with the anti-cytokine antibodies. This panel of anti-cytokine antibodies may be used to evaluate cytokine expression in snap-frozen tissue samples from the cetacean species tested.

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