Temperature-programmed high performance liquid chromatography separation of mono- and divinyl chlorophyll forms from marine phytoplankton

Divinyl chlorophyll a (a2) and divinyl chlorophyll b (b2) are chemotaxonomic marker pigments for the marine prochlorophytes, and can be used to study their distribution in marine samples. In this paper we report a baseline resolution of mono- and divinyl forms of chlorophylls a and b employing polym...

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Bibliographic Details
Published in:Chromatographia
Main Authors: Van Lenning, K., Garrido, J. L., Aristegui, J., Zapata, M.
Other Authors: 16481789200, 7202779797, 7006816204, 7005932573, 36472814900, 4153799, 680117, 227201, 5243361, WOS:VANLENNING, K, WOS:GARRIDO, JL, WOS:ARISTEGUI, J, WOS:ZAPATA, M
Format: Article in Journal/Newspaper
Language:English
Published: 0009-5893 1995
Subjects:
241705 Biología marina
Subtropical North-Atlantic
Prochlorococcus-Marinus
Hplc-Analysis
Pigments
Carotenoids
Column
Prochlorophyte
Prokaryote
Products
Ocean
North Atlantic
Online Access:http://hdl.handle.net/10553/51672
https://doi.org/10.1007/BF02688083
Description
Summary:Divinyl chlorophyll a (a2) and divinyl chlorophyll b (b2) are chemotaxonomic marker pigments for the marine prochlorophytes, and can be used to study their distribution in marine samples. In this paper we report a baseline resolution of mono- and divinyl forms of chlorophylls a and b employing polymeric ODS stationary phases at sub-ambient temperatures. The simultaneous resolution of mono- and divinyl forms of chlorophylls a and b, chlorophylls c1, c2, c3, Mg 3,8-divinylphaeoporphyrin a5 monomethyl ester (MgDVP), and phytol-substituted chlorophylls c was achieved when a temperature step gradient was employed during the analysis. An example is given of the utility of the protocol in oceanic field samples. 543 539

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