Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System
This study reports the synthesis of a hybrid sol-gel material, based on organically modified silanes (ORMOSILs) with epoxy functional groups, and its application in the stabilization of lipase type B from Candida antarctica (CalB) through sol-gel entrapment. The key immobilization parameters in the...
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Online Access: | http://hdl.handle.net/10553/118803 https://doi.org/10.3390/foods11162485 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/118803 2023-05-15T13:56:56+02:00 Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System Vasilescu, Corina Paul, Cristina Marc, Simona Hulka, Iosif Péter, Francisc orcid:0000-0002-3436-8327 orcid:0000-0001-5570-8418 orcid:0000-0003-1099-331X orcid:0000-0001-5442-0631 orcid:0000-0001-7248-2641 BU-ING 2022 http://hdl.handle.net/10553/118803 https://doi.org/10.3390/foods11162485 eng eng Foods 2304-8158 http://hdl.handle.net/10553/118803 doi:10.3390/foods11162485 2-s2.0-85137329085 WOS:000846142000001 16 Sí Foods [ISSN 2304-8158], v. 11 (16), 2485, (Julio 2022) 3206 Ciencias de la nutrición Candida antarctica B lipase (CalB) Sol-gel entrapment Response surface methodology (RSM) Green biocatalysis Food aroma ester production Continuous flow synthesis info:eu-repo/semantics/article 2022 ftunivlaspalmas https://doi.org/10.3390/foods11162485 2022-10-11T23:13:11Z This study reports the synthesis of a hybrid sol-gel material, based on organically modified silanes (ORMOSILs) with epoxy functional groups, and its application in the stabilization of lipase type B from Candida antarctica (CalB) through sol-gel entrapment. The key immobilization parameters in the sol-gel entrapment of lipase using epoxysilanes were optimized by the design of numerous experiments, demonstrating that glycidoxypropyl-trimethoxysilane can allow the formation of a matrix with excellent properties in view of the biocatalytic esterifications catalyzed by this lipase, at an enzyme loading of 25 g/mol of silane. The characterization of the immobilized biocatalyst and the correlation of its catalytic efficiency with the morphological and physicochemical properties of the sol-gel matrix was accomplished through scanning electron microscopy (SEM), fluorescence microscopy (FM), as well as thermogravimetric and differential thermal analysis (TGA/DTA). The operational and thermal stability of lipase were increased as a result of immobilization, with the entrapped lipase retaining 99% activity after 10 successive reaction cycles in the batch solventless synthesis of n-amyl caproate. A possible correlation of optimal productivity and yield was attempted for this immobilized lipase via the continuous flow synthesis of n-amyl caproate in a solventless system. The robustness and excellent biocatalytic efficiency of the optimized biocatalyst provide a promising solution for the synthesis of food-grade flavor esters, even at larger scales. 0,774 4,35 Q1 Q2 SCIE 10,5 Article in Journal/Newspaper Antarc* Antarctica Universidad de Las Palmas de Gran Canaria: Acceda Foods 11 16 2485 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
3206 Ciencias de la nutrición Candida antarctica B lipase (CalB) Sol-gel entrapment Response surface methodology (RSM) Green biocatalysis Food aroma ester production Continuous flow synthesis |
spellingShingle |
3206 Ciencias de la nutrición Candida antarctica B lipase (CalB) Sol-gel entrapment Response surface methodology (RSM) Green biocatalysis Food aroma ester production Continuous flow synthesis Vasilescu, Corina Paul, Cristina Marc, Simona Hulka, Iosif Péter, Francisc Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
topic_facet |
3206 Ciencias de la nutrición Candida antarctica B lipase (CalB) Sol-gel entrapment Response surface methodology (RSM) Green biocatalysis Food aroma ester production Continuous flow synthesis |
description |
This study reports the synthesis of a hybrid sol-gel material, based on organically modified silanes (ORMOSILs) with epoxy functional groups, and its application in the stabilization of lipase type B from Candida antarctica (CalB) through sol-gel entrapment. The key immobilization parameters in the sol-gel entrapment of lipase using epoxysilanes were optimized by the design of numerous experiments, demonstrating that glycidoxypropyl-trimethoxysilane can allow the formation of a matrix with excellent properties in view of the biocatalytic esterifications catalyzed by this lipase, at an enzyme loading of 25 g/mol of silane. The characterization of the immobilized biocatalyst and the correlation of its catalytic efficiency with the morphological and physicochemical properties of the sol-gel matrix was accomplished through scanning electron microscopy (SEM), fluorescence microscopy (FM), as well as thermogravimetric and differential thermal analysis (TGA/DTA). The operational and thermal stability of lipase were increased as a result of immobilization, with the entrapped lipase retaining 99% activity after 10 successive reaction cycles in the batch solventless synthesis of n-amyl caproate. A possible correlation of optimal productivity and yield was attempted for this immobilized lipase via the continuous flow synthesis of n-amyl caproate in a solventless system. The robustness and excellent biocatalytic efficiency of the optimized biocatalyst provide a promising solution for the synthesis of food-grade flavor esters, even at larger scales. 0,774 4,35 Q1 Q2 SCIE 10,5 |
author2 |
orcid:0000-0002-3436-8327 orcid:0000-0001-5570-8418 orcid:0000-0003-1099-331X orcid:0000-0001-5442-0631 orcid:0000-0001-7248-2641 BU-ING |
format |
Article in Journal/Newspaper |
author |
Vasilescu, Corina Paul, Cristina Marc, Simona Hulka, Iosif Péter, Francisc |
author_facet |
Vasilescu, Corina Paul, Cristina Marc, Simona Hulka, Iosif Péter, Francisc |
author_sort |
Vasilescu, Corina |
title |
Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
title_short |
Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
title_full |
Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
title_fullStr |
Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
title_full_unstemmed |
Development of a Tailored Sol-Gel Immobilized Biocatalyst for Sustainable Synthesis of the Food Aroma Ester n-Amyl Caproate in Continuous Solventless System |
title_sort |
development of a tailored sol-gel immobilized biocatalyst for sustainable synthesis of the food aroma ester n-amyl caproate in continuous solventless system |
publishDate |
2022 |
url |
http://hdl.handle.net/10553/118803 https://doi.org/10.3390/foods11162485 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Foods [ISSN 2304-8158], v. 11 (16), 2485, (Julio 2022) |
op_relation |
Foods 2304-8158 http://hdl.handle.net/10553/118803 doi:10.3390/foods11162485 2-s2.0-85137329085 WOS:000846142000001 16 Sí |
op_doi |
https://doi.org/10.3390/foods11162485 |
container_title |
Foods |
container_volume |
11 |
container_issue |
16 |
container_start_page |
2485 |
_version_ |
1766264532735361024 |